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Vladimir Shirokov
Vladimir Shirokov
在 vega.protres.ru 的電子郵件地址已通過驗證
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引用次數
引用次數
年份
Crystallization of 70 S ribosomes and 30 S ribosomal subunits from Thermus thermophilus
SD Trakhanov, MM Yusupov, SC Agalarov, MB Garber, SN Ryazantsev, ...
Febs Letters 220 (2), 319-322, 1987
1271987
Membrane protein expression in cell-free systems
B Schneider, F Junge, VA Shirokov, F Durst, D Schwarz, V Dötsch, ...
Heterologous Expression of Membrane Proteins: Methods and Protocols, 165-186, 2010
1152010
Cell-free production of biologically active polypeptides: application to the synthesis of antibacterial peptide cecropin
KA Martemyanov, VA Shirokov, OV Kurnasov, AT Gudkov, AS Spirin
Protein expression and Purification 21 (3), 456-461, 2001
1142001
Step-wise formation of eukaryotic double-row polyribosomes and circular translation of polysomal mRNA
GS Kopeina, ZA Afonina, KV Gromova, VA Shirokov, VD Vasiliev, ...
Nucleic acids research 36 (8), 2476-2488, 2008
962008
Continuous-exchange protein-synthesizing systems
VA Shirokov, A Kommer, VA Kolb, AS Spirin
In Vitro Transcription and Translation Protocols, 19-55, 2007
712007
Preliminary X-ray investigation of 70 S ribosome crystals from Thermus thermophilus
S Trakhanov, M Yusupov, V Shirokov, M Garber, A Mitschler, M Ruff, ...
Journal of molecular biology 209 (2), 327-328, 1989
661989
The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes.
AG Myasnikov, ZA Afonina, JF Ménétret, VA Shirokov, AS Spirin, ...
Nature communications 5, 5294-5294, 2014
572014
Strategies for the cell-free expression of membrane proteins
S Reckel, S Sobhanifar, F Durst, F Löhr, VA Shirokov, V Dötsch, ...
Cell-Free Protein Production: Methods and Protocols, 187-212, 2010
572010
Formation of circular polyribosomes on eukaryotic mRNA without cap-structure and poly (A)-tail: a cryo electron tomography study
ZA Afonina, AG Myasnikov, VA Shirokov, BP Klaholz, AS Spirin
Nucleic acids research 42 (14), 9461-9469, 2014
562014
Conformation transitions of eukaryotic polyribosomes during multi-round translation
ZA Afonina, AG Myasnikov, VA Shirokov, BP Klaholz, AS Spirin
Nucleic acids research 43 (1), 618-628, 2014
472014
Continuous-exchange cell-free protein-synthesizing system: synthesis of HIV-1 antigen Nef
MN Chekulayeva, OV Kurnasov, VA Shirokov, AS Spirin
Biochemical and biophysical research communications 280 (3), 914-917, 2001
412001
Crystallization of protein and ribosomal particles in microgravity
SD Trakhanov, AI Grebenko, VA Shirokov, AV Gudkov, AV Egorov, ...
Journal of crystal growth 110 (1-2), 317-321, 1991
321991
Topology of mRNA chain in isolated eukaryotic double-row polyribosomes
ZA Afonina, AG Myasnikov, NF Khabibullina, AY Belorusova, JF Menetret, ...
Biochemistry (Moscow) 78 (5), 445-454, 2013
242013
Effective non‐viral leader for cap‐independent translation in a eukaryotic cell‐free system
LA Shaloiko, IE Granovsky, TV Ivashina, VN Ksenzenko, VA Shirokov, ...
Biotechnology and bioengineering 88 (6), 730-739, 2004
212004
Purification and crystallization of components of the protein-synthesizing system from Thermus thermophilus
MB Garber, SC Agalarov, IA Eliseikina, SE Sedelnikova, SV Tishchenko, ...
Journal of crystal growth 110 (1-2), 228-236, 1991
181991
Method of preparing polypeptides in cell-free system and device for its realization
SV Biryukov, PN Simonenko, VA Shirokov, SG Mayorov, AS Spirin
US Patent 6,518,058, 2003
162003
Method for synthesis of polypeptides in cell-free systems
SV Biryukov, PN Simonenko, VA Shirokov, AS Spirin
US Patent 6,783,957, 2004
132004
Continuous-flow and continuous-exchange cell-free translation systems and reactors
VA Shirokov, PN Simonenko, SV Biryukov, AS Spirin
Cell-Free Translation Systems, 91-107, 2002
132002
Undecagold cluster modified tRNAPhe from Escherichia coli and its activity in the protein elongation cycle
B BLECHSCHMIDT, V SHIROKOV, M SPRINZL
The FEBS Journal 219 (1‐2), 65-71, 1994
101994
Thin laminar microcrystals of 70 S ribosomes from Thermus thermophilus
VA Shirokov, SN Ryazantsev, YV Sergeev, MB Garber, VD Vasiliev
FEBS letters 258 (1), 147-149, 1989
71989
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