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Membrane protein expression in cell-free systems B Schneider, F Junge, VA Shirokov, F Durst, D Schwarz, V Dötsch, ... Heterologous Expression of Membrane Proteins: Methods and Protocols, 165-186, 2010 | 115 | 2010 |
Cell-free production of biologically active polypeptides: application to the synthesis of antibacterial peptide cecropin KA Martemyanov, VA Shirokov, OV Kurnasov, AT Gudkov, AS Spirin Protein expression and Purification 21 (3), 456-461, 2001 | 114 | 2001 |
Step-wise formation of eukaryotic double-row polyribosomes and circular translation of polysomal mRNA GS Kopeina, ZA Afonina, KV Gromova, VA Shirokov, VD Vasiliev, ... Nucleic acids research 36 (8), 2476-2488, 2008 | 96 | 2008 |
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The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes. AG Myasnikov, ZA Afonina, JF Ménétret, VA Shirokov, AS Spirin, ... Nature communications 5, 5294-5294, 2014 | 57 | 2014 |
Strategies for the cell-free expression of membrane proteins S Reckel, S Sobhanifar, F Durst, F Löhr, VA Shirokov, V Dötsch, ... Cell-Free Protein Production: Methods and Protocols, 187-212, 2010 | 57 | 2010 |
Formation of circular polyribosomes on eukaryotic mRNA without cap-structure and poly (A)-tail: a cryo electron tomography study ZA Afonina, AG Myasnikov, VA Shirokov, BP Klaholz, AS Spirin Nucleic acids research 42 (14), 9461-9469, 2014 | 56 | 2014 |
Conformation transitions of eukaryotic polyribosomes during multi-round translation ZA Afonina, AG Myasnikov, VA Shirokov, BP Klaholz, AS Spirin Nucleic acids research 43 (1), 618-628, 2014 | 47 | 2014 |
Continuous-exchange cell-free protein-synthesizing system: synthesis of HIV-1 antigen Nef MN Chekulayeva, OV Kurnasov, VA Shirokov, AS Spirin Biochemical and biophysical research communications 280 (3), 914-917, 2001 | 41 | 2001 |
Crystallization of protein and ribosomal particles in microgravity SD Trakhanov, AI Grebenko, VA Shirokov, AV Gudkov, AV Egorov, ... Journal of crystal growth 110 (1-2), 317-321, 1991 | 32 | 1991 |
Topology of mRNA chain in isolated eukaryotic double-row polyribosomes ZA Afonina, AG Myasnikov, NF Khabibullina, AY Belorusova, JF Menetret, ... Biochemistry (Moscow) 78 (5), 445-454, 2013 | 24 | 2013 |
Effective non‐viral leader for cap‐independent translation in a eukaryotic cell‐free system LA Shaloiko, IE Granovsky, TV Ivashina, VN Ksenzenko, VA Shirokov, ... Biotechnology and bioengineering 88 (6), 730-739, 2004 | 21 | 2004 |
Purification and crystallization of components of the protein-synthesizing system from Thermus thermophilus MB Garber, SC Agalarov, IA Eliseikina, SE Sedelnikova, SV Tishchenko, ... Journal of crystal growth 110 (1-2), 228-236, 1991 | 18 | 1991 |
Method of preparing polypeptides in cell-free system and device for its realization SV Biryukov, PN Simonenko, VA Shirokov, SG Mayorov, AS Spirin US Patent 6,518,058, 2003 | 16 | 2003 |
Method for synthesis of polypeptides in cell-free systems SV Biryukov, PN Simonenko, VA Shirokov, AS Spirin US Patent 6,783,957, 2004 | 13 | 2004 |
Continuous-flow and continuous-exchange cell-free translation systems and reactors VA Shirokov, PN Simonenko, SV Biryukov, AS Spirin Cell-Free Translation Systems, 91-107, 2002 | 13 | 2002 |
Undecagold cluster modified tRNAPhe from Escherichia coli and its activity in the protein elongation cycle B BLECHSCHMIDT, V SHIROKOV, M SPRINZL The FEBS Journal 219 (1‐2), 65-71, 1994 | 10 | 1994 |
Thin laminar microcrystals of 70 S ribosomes from Thermus thermophilus VA Shirokov, SN Ryazantsev, YV Sergeev, MB Garber, VD Vasiliev FEBS letters 258 (1), 147-149, 1989 | 7 | 1989 |